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Enzo Biochem soluble cd40l (scd40l
Soluble Cd40l (Scd40l, supplied by Enzo Biochem, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/soluble+cd40l+(scd40l/soluble+cd40l/10__7554_slash_elife__30442-233-13-16
Average 90 stars, based on 1 article reviews
soluble cd40l (scd40l - by Bioz Stars, 2026-09
90/100 stars

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Related Articles

Isolation:

Article Title: Method to induce and expand therapeutic alloantigen-specific human regulatory T cells in large-scale
Article Snippet: .. Alternatively, t-CD40L cells were replaced by different concentrations of the soluble hexameric CD40L (sCD40L, Alexis Biochemicals, Switzerland) to expand B cells as above.30 T-cell Isolation Human CD4+ or naïve CD4+ T cells were isolated from healthy donor PBMC by negative selection using a CD4+ T-cell isolation kit or a naïve CD4+ T-cell isolation kit (Miltenyi Biotec, Calif.) for depletion of cells expressing CD8, CD 14, CD 16, CD 19, CD36, CD56, CD123, TCRγ/δ and CD235a (glycophorin A) (for CD4+T cells) or depletion of CD8, CD14, CD16, CD19, CD36, CD56, CD123, TCRγ/δ, CD235a and CD45RO (for naïve CD4+ T cells). .. The CD25+ cells were further depleted by positive selection with directly conjugated anti-CD25 magnetic microbeads (Miltenyi Biotec, Calif.) following the double-column depletion procedures.

Article Title: Method to induce and expand therapeutic alloantigen-specific human regulatory T cells in large-scale
Article Snippet: .. Alternatively, t-CD40L cells were replaced by different concentrations of the soluble hexameric CD40L (sCD40L, Alexis Biochemicals, Switzerland) to expand B cells as above.30 T-cell Isolation Human CD4+ or naïve CD4+ T cells were isolated from healthy donor PBMC by negative selection using a CD4+ T-cell isolation kit or a naïve CD4+ T-cell isolation kit (Miltenyi Biotec, Calif.) for depletion of cells expressing CD8, CD14, CD 16, CD 19, CD36, CD56, CD123, TCRγ/δ and CD235a (glycophorin A) (for CD4+ T cells) or depletion of CD8, CD14, CD16, CD19, CD36, CD56, CD123, TCR-γ/δ, CD235a and CD45RO (for naïve CD4+ T cells). .. The CD25+ cells were further depleted by positive selection with directly conjugated anti-CD25 magnetic microbeads (Miltenyi Biotec, Calif.) following the double-column depletion procedures.

Article Title: Promoter methylation of argininosuccinate synthetase-1 sensitises lymphomas to arginine deiminase treatment, autophagy and caspase-dependent apoptosis
Article Snippet: Chloroquine (25 μ M, Sigma-Aldrich) was added with ADI-PEG20 to assess the effect on LC3 protein turnover and lymphoid cell line viability. .. Isolated primary B lymphoma cells and CTCL (Sezary syndrome) cells, obtained using informed patient consent and Research Ethics Committee approval, were suspended in freshly prepared culture medium (IMDM; Gibco Biocult, Paisley, UK, including 10% human serum) at a concentration of 5 × 10 5 cells/ml, and plated into 96-well plates with the addition of soluble recombinant human CD40L (sCD40L at 100 ng/ml, Enzo Life Sciences, Exeter, UK) and recombinant interleukin-4 (IL-4 at 5 ng/ml, Sigma-Aldrich Co., UK) for 24 h before treatment with ADI-PEG20 for a further 72 h. The effect of chloroquine (25 μ M) and doxorubicin (1 μ M, a positive control) was assessed also, and cell viability was measured using the ViaLight assay (Lonza, Rockland, ME, USA). ..

Selection:

Article Title: Method to induce and expand therapeutic alloantigen-specific human regulatory T cells in large-scale
Article Snippet: .. Alternatively, t-CD40L cells were replaced by different concentrations of the soluble hexameric CD40L (sCD40L, Alexis Biochemicals, Switzerland) to expand B cells as above.30 T-cell Isolation Human CD4+ or naïve CD4+ T cells were isolated from healthy donor PBMC by negative selection using a CD4+ T-cell isolation kit or a naïve CD4+ T-cell isolation kit (Miltenyi Biotec, Calif.) for depletion of cells expressing CD8, CD 14, CD 16, CD 19, CD36, CD56, CD123, TCRγ/δ and CD235a (glycophorin A) (for CD4+T cells) or depletion of CD8, CD14, CD16, CD19, CD36, CD56, CD123, TCRγ/δ, CD235a and CD45RO (for naïve CD4+ T cells). .. The CD25+ cells were further depleted by positive selection with directly conjugated anti-CD25 magnetic microbeads (Miltenyi Biotec, Calif.) following the double-column depletion procedures.

Article Title: Method to induce and expand therapeutic alloantigen-specific human regulatory T cells in large-scale
Article Snippet: .. Alternatively, t-CD40L cells were replaced by different concentrations of the soluble hexameric CD40L (sCD40L, Alexis Biochemicals, Switzerland) to expand B cells as above.30 T-cell Isolation Human CD4+ or naïve CD4+ T cells were isolated from healthy donor PBMC by negative selection using a CD4+ T-cell isolation kit or a naïve CD4+ T-cell isolation kit (Miltenyi Biotec, Calif.) for depletion of cells expressing CD8, CD14, CD 16, CD 19, CD36, CD56, CD123, TCRγ/δ and CD235a (glycophorin A) (for CD4+ T cells) or depletion of CD8, CD14, CD16, CD19, CD36, CD56, CD123, TCR-γ/δ, CD235a and CD45RO (for naïve CD4+ T cells). .. The CD25+ cells were further depleted by positive selection with directly conjugated anti-CD25 magnetic microbeads (Miltenyi Biotec, Calif.) following the double-column depletion procedures.

Expressing:

Article Title: Method to induce and expand therapeutic alloantigen-specific human regulatory T cells in large-scale
Article Snippet: .. Alternatively, t-CD40L cells were replaced by different concentrations of the soluble hexameric CD40L (sCD40L, Alexis Biochemicals, Switzerland) to expand B cells as above.30 T-cell Isolation Human CD4+ or naïve CD4+ T cells were isolated from healthy donor PBMC by negative selection using a CD4+ T-cell isolation kit or a naïve CD4+ T-cell isolation kit (Miltenyi Biotec, Calif.) for depletion of cells expressing CD8, CD 14, CD 16, CD 19, CD36, CD56, CD123, TCRγ/δ and CD235a (glycophorin A) (for CD4+T cells) or depletion of CD8, CD14, CD16, CD19, CD36, CD56, CD123, TCRγ/δ, CD235a and CD45RO (for naïve CD4+ T cells). .. The CD25+ cells were further depleted by positive selection with directly conjugated anti-CD25 magnetic microbeads (Miltenyi Biotec, Calif.) following the double-column depletion procedures.

Article Title: Method to induce and expand therapeutic alloantigen-specific human regulatory T cells in large-scale
Article Snippet: .. Alternatively, t-CD40L cells were replaced by different concentrations of the soluble hexameric CD40L (sCD40L, Alexis Biochemicals, Switzerland) to expand B cells as above.30 T-cell Isolation Human CD4+ or naïve CD4+ T cells were isolated from healthy donor PBMC by negative selection using a CD4+ T-cell isolation kit or a naïve CD4+ T-cell isolation kit (Miltenyi Biotec, Calif.) for depletion of cells expressing CD8, CD14, CD 16, CD 19, CD36, CD56, CD123, TCRγ/δ and CD235a (glycophorin A) (for CD4+ T cells) or depletion of CD8, CD14, CD16, CD19, CD36, CD56, CD123, TCR-γ/δ, CD235a and CD45RO (for naïve CD4+ T cells). .. The CD25+ cells were further depleted by positive selection with directly conjugated anti-CD25 magnetic microbeads (Miltenyi Biotec, Calif.) following the double-column depletion procedures.

other:

Article Title: Autoantibody-producing RP105(-) B cells, from patients with systemic lupus erythematosus, showed more preferential expression of BCMA compared with BAFF-R than normal subjects.
Article Snippet: Recombinant human soluble BAFF (rhsBAFF; produced in bacteria, extracellular domain of human BAFF, amino acid 83–285, is fused at the N-terminus to a linker peptide), recombinant human APRIL (rhsMegaAPRIL; extracellular domain of human APRIL, amino acid 105–250, is fused at the N-terminus), recombinant human soluble CD40L (sCD40L) and enhancer were also obtained from ALEXIS Biochemicals (San Diego, CA, USA).

Article Title: CD40 is a major regulator of dendrite growth from developing excitatory and inhibitory neurons
Article Snippet: The cultures were treated with the following reagents as indicated in the text: soluble CD40L (sCD40L) (Enzo Life Sciences, Farmingdale, NY, USA, ALX-522–120 C010), CD40-Fc (Enzo Life Sciences, ALX-522–016 C050), Fc protein (Enzo Life Sciences, ALX-203–004 C050), Go6983 (Tocris Biosciences, UK, cat. no. 2285) and phorbol-12-myristate-13-acetate (PMA) (MERCK, UK, cat. no. 524400).

Article Title: High serum levels of BAFF, APRIL and TACI in Common Variable Immunodeficiency
Article Snippet: As additional stimulators in other experiments, cells were also cultured with 100 ng/ml lipopolysaccharide (LPS) (Sigma, St Louis MO) or 300 ng/ml soluble CD40L (sCD40L) fusion protein, (Alexis, Axxora LLC, San Diego, CA.)

Article Title: Soluble CD40 ligand directly alters glomerular permeability and may act as a circulating permeability factor in FSGS
Article Snippet: Human recombinant soluble CD40L (hr-sCD40L) trimeric protein plus enhancer (cross-linking Ab), mouse recombinant soluble CD40L (mr-sCD40L) set, recombinant CD40-murine Ig (muIg) fusion protein, consisting of the extracellular domain of human CD40 fused to mouse IgG2a, recombinant human CD40L-muCD8 fusion protein, constituted by murine CD8 fused to human CD40L, and anti-human CD40L mAbs (MK13A4 and 24–31) were obtained from Alexis Biochemicals (San Diego, CA).

Incubation:

Article Title: Effects of CD40 binding on ovarian carcinoma cell growth and cytokine production in vitro
Article Snippet: .. In some experiments, OVCA cells were incubated with 1 μg/ml soluble recombinant human CD40L (sCD40L; Alexis Biochemicals, Coger, France). ..

Recombinant:

Article Title: Effects of CD40 binding on ovarian carcinoma cell growth and cytokine production in vitro
Article Snippet: .. In some experiments, OVCA cells were incubated with 1 μg/ml soluble recombinant human CD40L (sCD40L; Alexis Biochemicals, Coger, France). ..

Article Title: Promoter methylation of argininosuccinate synthetase-1 sensitises lymphomas to arginine deiminase treatment, autophagy and caspase-dependent apoptosis
Article Snippet: Chloroquine (25 μ M, Sigma-Aldrich) was added with ADI-PEG20 to assess the effect on LC3 protein turnover and lymphoid cell line viability. .. Isolated primary B lymphoma cells and CTCL (Sezary syndrome) cells, obtained using informed patient consent and Research Ethics Committee approval, were suspended in freshly prepared culture medium (IMDM; Gibco Biocult, Paisley, UK, including 10% human serum) at a concentration of 5 × 10 5 cells/ml, and plated into 96-well plates with the addition of soluble recombinant human CD40L (sCD40L at 100 ng/ml, Enzo Life Sciences, Exeter, UK) and recombinant interleukin-4 (IL-4 at 5 ng/ml, Sigma-Aldrich Co., UK) for 24 h before treatment with ADI-PEG20 for a further 72 h. The effect of chloroquine (25 μ M) and doxorubicin (1 μ M, a positive control) was assessed also, and cell viability was measured using the ViaLight assay (Lonza, Rockland, ME, USA). ..

Concentration Assay:

Article Title: Promoter methylation of argininosuccinate synthetase-1 sensitises lymphomas to arginine deiminase treatment, autophagy and caspase-dependent apoptosis
Article Snippet: Chloroquine (25 μ M, Sigma-Aldrich) was added with ADI-PEG20 to assess the effect on LC3 protein turnover and lymphoid cell line viability. .. Isolated primary B lymphoma cells and CTCL (Sezary syndrome) cells, obtained using informed patient consent and Research Ethics Committee approval, were suspended in freshly prepared culture medium (IMDM; Gibco Biocult, Paisley, UK, including 10% human serum) at a concentration of 5 × 10 5 cells/ml, and plated into 96-well plates with the addition of soluble recombinant human CD40L (sCD40L at 100 ng/ml, Enzo Life Sciences, Exeter, UK) and recombinant interleukin-4 (IL-4 at 5 ng/ml, Sigma-Aldrich Co., UK) for 24 h before treatment with ADI-PEG20 for a further 72 h. The effect of chloroquine (25 μ M) and doxorubicin (1 μ M, a positive control) was assessed also, and cell viability was measured using the ViaLight assay (Lonza, Rockland, ME, USA). ..

Positive Control:

Article Title: Promoter methylation of argininosuccinate synthetase-1 sensitises lymphomas to arginine deiminase treatment, autophagy and caspase-dependent apoptosis
Article Snippet: Chloroquine (25 μ M, Sigma-Aldrich) was added with ADI-PEG20 to assess the effect on LC3 protein turnover and lymphoid cell line viability. .. Isolated primary B lymphoma cells and CTCL (Sezary syndrome) cells, obtained using informed patient consent and Research Ethics Committee approval, were suspended in freshly prepared culture medium (IMDM; Gibco Biocult, Paisley, UK, including 10% human serum) at a concentration of 5 × 10 5 cells/ml, and plated into 96-well plates with the addition of soluble recombinant human CD40L (sCD40L at 100 ng/ml, Enzo Life Sciences, Exeter, UK) and recombinant interleukin-4 (IL-4 at 5 ng/ml, Sigma-Aldrich Co., UK) for 24 h before treatment with ADI-PEG20 for a further 72 h. The effect of chloroquine (25 μ M) and doxorubicin (1 μ M, a positive control) was assessed also, and cell viability was measured using the ViaLight assay (Lonza, Rockland, ME, USA). ..



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